ABSTRACT
Objective: To investigate the effects of the active ingredients combined therapy on inflammatory factors interleukin 1 beta (IL-1β) and neuropeptide Y (NPY) based onpharmacodynamics in rats. Methods: The animal model was built by transient middle cerebral artery occlusion (MCAO). The method for evaluating the concentrations of the FA-Pr-Al components in rat plasma was established by using HPLC and the expression levels of IL-1βand NPY were determined by ELISA. A new mathematics method of the trend of percentage rate of change (PRC) was used to assess the correlation between pharmacokinetics (PK) and pharmacodynamics (PD). Results: FA-Pr-Al in combination reduced neurological deficits, decreased infarct volume and inhibited the expression levels of IL-1βand NPY (all P<0.05)compared with the model group. FA, Pr and Al all displayed two compartment open models in rats. Clockwise hysteresis loops were obtained by time-concentration-effect curves. IL-1βand NPY level changes in the plasma followed an opposite trend to the plasma concentration tendency after Cmax was reached. Astragaloside’s PRC value was significantly higher than those of FA and puerarin between 120 to 180 min. Conclusions: The pharmacokinetics of FA-Pr-Al in combination were closely related its pharmacodynamics in treating ischemia/reperfusion injury, and the components of FA-Pr-Al may have a synergistic pharmacological effect. Astragaloside may play a more pronounced role in regulating IL-1毬and NPY levels comparedwith puerarin or FA.
ABSTRACT
Objective Explore the regulation of haw and Red Sage on blood lipid. Method Use high-lipid forage to make animal model, the bland group of 8 animals were administered with general forage(blank control); the high-lipid forage group of 42 with high-lipid forage; the model-making group was divided randomly into 5 groups, i.e. model, Shujiang, haw, Red Sage, haw and Red Sage groups. After 14 days, respectively measure serum TC,TG,HDL-C,LDL-C contents, as well as plasma SOD activity. Result Haw and Red Sage in combination can reduce TC,TG, LDL, raise HDL contents, and markedly lower SOD activity. Conclusion Haw and Red Sage in combination can function to regulate blood lipid.